《植物生理学报》 2012, 48(3): 282-288

新型甘蓝型油菜温敏核不育系SP2S的花药发育解剖学观察

郭英芬¹, 葛娟¹, 于澄宇^1,*, 张国云², 董军刚¹, 董振生^1,*

西北农林科技大学1农学院, 2大型仪器设备管理中心, 陕西杨凌712100

通信作者：于澄宇；E-mail: yu1009@nwsuaf.edu.cn, dzs05319@163.com；Tel: 029-87082854)

摘　要：

SP2S是西北农林科技大学选育的甘蓝型油菜温敏核不育系, 本文采用半薄树脂切片、扫描电镜对SP2S及其可育近等基因系SP2F的花药发育及花粉形态进行观察比较, 发现SP2S花药发育在减数分裂时期出现异常, 单核花粉时期彻底败育。其主要特征是: 减数分裂时期绒毡层已经径向肥大且出现大液泡, 胼胝质不能及时降解, 使得单核小孢子相互粘连在一起, 小孢子无花粉壁的形成且细胞质物质逐渐降解, 最后小孢子仅剩下空壳残留物, 聚集在一起。SP2S败育特征与现有的核不育材料不同, 表明其有可能是一种新型温敏核不育材料。

关键词：甘蓝型油菜; 光温敏不育; 花药败育; 解剖学观察; 小孢子

收稿：2011-11-29   修定：2011-12-28

资助：国家自然科学基金(31071454)、教育部新世纪优秀人才项目(NCET-10-0693)和中央高校基本科研业务费专项(QN2009008)。

Anatomical Observation of Anther Development of a New Thermo-Sensitive Genic Male Sterile Line SP2S in Brassica napus L.

GUO Ying-Fen¹, GE Juan¹, YU Cheng-Yu^1,*, ZHANG Guo-Yun², DONG Jun-Gang¹, DONG Zhen-Sheng^1,*

¹College of Agronomy, ²Management Centre for Large Equipment, Northwest A&F University, Yangling, Shaanxi 712100, China

Corresponding author: YU Cheng-Yu; E-mail: yu1009@nwsuaf.edu.cn, dzs05319@163.com; Tel: 029-87082854)

Abstract:

SP2S is a new type thermo-sensitive genic male sterile line of Brassica napus breeding in Northwest A&F University. The anatomical characters of pollen and the anther development of SP2S at different stages were compared with its near isogenic line SP2F by semi-thin sections and scan electronic microscopy. The result indicated that the anther abortion occurred at the stage of meiosis and the anther completely aborted at the later stage of uni-nucleate. The tapetal cells were highly vacuolated and deformed at the stage of meiosis. Callose around the tetrads couldn’t be degradated timely, which made the microspores adhesion with each other. And then the microspores didn’t form the extine normally. The cytoplasm of pollen cells decayed gradually and at last became empty. These evidences obviously indicate that SP2S is probably a new thermosensitive male sterile line which is different from the previously materials.

Key words: Brassica napus L; thermo-sensitive male sterility; anther abortion; anatomical observation; microspore